But the random ascospore progeny may be contaminated by conidia while conducting single ascus and ascospore isolation, which may result in a genetic bias. 采用先分离单个子囊，待其长成菌落并产生分生孢子后，再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。
The variation range of leaf length, width, area, and thickness and stoma length, width, density of the sexual progenies were crisscrossed with their parents. 有性后代除叶厚变异范围较小外，其它参数变异范围都较大。
