Five zones were separated by improved discontinuous sucrose gradient centrifugation. 用改进的不连续蔗糖密度梯度离心法分离到五条带。
Methods Using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared. 方法采用不同的胶原酶消化法及不连续密度梯度纯化法。